Yes, Immobilon FL can be used as an alternative to Immobilon P for western blots.
IPFL00010
Immobilon® -FL PVDF Membrane
Immobilon®-FL PVDF Membrane
1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane with low background fluorescence
Synonym(s):
Western blotting membrane, blotting membrane, transfer membrane
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1 EA
CZK 16,800.00
CZK 16,800.00
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1 EA
CZK 16,800.00
About This Item
UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
material:
PVDF membrane
plain filter
white filter
plain filter
white filter
detection method:
chemiluminescent
colorimetric
fluorometric
colorimetric
fluorometric
pore size:
0.45 μm pore size
compatibility:
for use with All fluorescence dyes
for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
CZK 16,800.00
In StockDetails
Recommended Products
Product Name
Immobilon®-FL PVDF Membrane, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
material
PVDF membrane
plain filter
white filter
Quality Level
feature
hydrophobic
manufacturer/tradename
Immobilon®
technique(s)
dot blot: suitable
western blot: suitable
filter L × W
27 cm × 3.75 m
pore size
0.45 μm pore size
capacity
155 μg/cm2 adsorption capacity (insulin)
205 μg/cm2 adsorption capacity (BSA)
300 μg/cm2 adsorption capacity (goat IgG)
compatibility
for use with All fluorescence dyes
for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
detection method
chemiluminescent
colorimetric
fluorometric
shipped in
ambient
Related Categories
General description
The Immobilon®-FL transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 μm. It is designed for binding proteins transferred from a variety of gel matrices. The Immobilon®-FL transfer membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane offers optimal blotting for proteins with molecular weights greater than 20,000. The Immobilon®-FL membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. Immobilon®-FL membrane can also be used for standard chemiluminescent or chromogenic detection.
Features and Benefits
Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications; Extremely low background enhances the sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at a wide range of excitation/emission wavelengths
- Best for multiplexing and chemifluorescence detections
- compatible with standard blocking agents and buffers
Legal Information
Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
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Is it possible to use Immobilon FL instead of Immobilon P for western blots?
1 answer-
Helpful?
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Is 0.22 µm PVDF membrane available for fluorescent detection in Western blotting?
1 answer-
Immobilon PSQ, with a pore size of 0.2 µm, is suitable for various detection methods including fluorescent, radioactive, chromogenic, and chemifluorescent detection. But when using Immobilon PSQ in Western Blotting, it is important to consider that there may be increased background noise, which might require more stringent wash steps to ensure accurate results.
Helpful?
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Active Filters
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Great for western blots
I have been using this product for close to two and half years now. It is a highly reliable PVDF roll for western blotting. I use it to transfer a wide variety of proteins and use HRP conjugated secondary antibodies for imaging bands. The results have always been sharp, reliable and most importantly, reproducible. I shall buy again when we run out.
- Received an incentive for this review
- No
Recommends this productYesHelpful?
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