GF333

IL-2,
Human Recombinant Animal Free

• Synonym(s): Interleukin-2, IL2
• UNSPSC Code: 12352202
• NACRES: NA.77
• eCl@ss: 32160405

Properties

• impurities: <0.1 μg/mg protein endotoxin (<1 EU/μg)
• Quality Level: 100
• UniProt accession no.: P60568
• shipped in: wet ice
• storage temp.: -10 to -25°C

Description

General description

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes.
Recombinant human IL-2 is a 15.5kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.

Product Source: Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.

Application

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Physical form

Product is presented without additives and is filtered through a 0.2 micron filter before lyophilization.

Preparation Note

Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in 100mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.

Analysis Note

Determined its its ability to stimulate the proliferation of mouse CTLL-2 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable