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EZRL

Rat Leptin ELISA Kit

measures and quantifies Leptin levels in 10 μL plasma or serum

Synonym(s):

Leptin, Obesity Factor

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96 wells

Available to ship TODAYfromAreál Kühne+Nagel spol. s r.o.

CZK 19,000.00
96 well

Available to ship TODAYfromAreál Kühne+Nagel spol. s r.o.

CZK 123,800.00

About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32
Input:
sample type plasma (K2 EDTA)
sample type serum
Species reactivity:
rat
Storage temp.:
2-8°C
Shipped in:
wet ice

CZK 19,000.00

Available to ship TODAYDetails

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Product Name

Rat Leptin ELISA, This Rat Leptin ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine research.

Quality Level

200

species reactivity

rat

packaging

kit of 1 × 96 wells

parameter

10 μL sample volume (4hr assay)

assay range

accuracy: 102-122%, linearity: 87-100%, sensitivity: 0.04 ng/mL

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type serum

UniProt accession no.

P41160, P50596

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

General description

Leptin is an adipokine secreted by the adipose tissue. It is also found in the primary and secondary lymphoid organs. Leptin has a 3D structure made up of four α-helix bundle motifs. It participates in regulating neuroendocrine function and energy homeostasis. Leptin also participates in physiological processes such as reproduction, angiogenesis, and hemopoiesis.[1] Rat Leptin enzyme-linked immunosorbent assay (ELISA) kit is used for the non-radioactive quantification of leptin in rat sera. Plasma samples may also be used but application to samples of other biological fluids may need validation by the user.

Application

Rat Leptin ELISA kit has been used in the determination of leptin from rat serum[2][3] and plasma samples[4].

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species reactivity

rat

species reactivity

mouse

species reactivity

human

species reactivity

rat, mouse

input

sample type plasma (K2 EDTA)
sample type serum

input

sample type serum
sample type plasma (K2 EDTA)

input

sample type cell culture supernatant
sample type plasma (K2 EDTA)
sample type serum

input

sample type serum
sample type plasma (K2 EDTA)

assay range

accuracy: 102-122%, sensitivity: 0.04 ng/mL, linearity: 87-100%

assay range

accuracy: 87-100%, linearity: 100-111%, sensitivity: 0.05 ng/mL, standard curve range: 0.2-30 ng/mL

assay range

accuracy: 94-114%, intra-assay cv: 1.4-4.9
(Sensitive Assay), intra-assay cv: 2.6-4.6, linearity: 80-105%, linearity: 96-125%
(sensitive assay), sensitivity: 0.125 ng/mL
(sensitive assay), sensitivity: 0.125-20 ng/mL, sensitivity: 0.5 ng/mL, standard curve range: 0.5-100 ng/mL

assay range

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric (450nm/590nm)

detection method

colorimetric (450nm/590nm)

detection method

colorimetric (450nm/590nm)

detection method

colorimetric (450nm/590nm)

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Warning

hcodes

H290,H317

Hazard Classifications

Met. Corr. 1 - Skin Sens. 1

wgk

WGK 3

Storage Class

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
EZRL-83BK04053252437045
EZRL-83K08436037125270

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