CC1043

MMP-14, human, prodomain, catalytic domain, and hemopexin domain, E. coli recombinant

• Synonym(s): MT1-MMP
• UNSPSC Code: 12352204
• NACRES: NA.41
• eCl@ss: 32160405
• Biological source: human
• Concentration: 0.2 μg/μL

Properties

• biological source: human
• Quality Level: 100
• form: liquid
• manufacturer/tradename: Chemicon^®
• concentration: 0.2 μg/μL
• NCBI accession no.: NM_004995.2
• UniProt accession no.: P50281
• shipped in: dry ice
• storage temp.: -65 to -96°C
• Gene Information: human ... MMP14(4323)

Description

General description

BACKGROUND: Matrix metalloproteinases (MMPs) are Zn2+- and Ca2+-dependent endopeptidases which function in the turnover of extracellular matrix components [Matrisian, 1992]. Presently, eighteen secreted MMPs and five membrane-type MMPs [Sato et al., 1994; Will & Hinzmann, 1995; Takino et al., 1995; Puente et al., 1996] are known to be expressed in vertebrates. Human MT1-MMP consists of 559 amino acid residues with a calculated Mr of 63516 [Sato et al., 1994; Will & Hinzmann, 1995]. The following domains and sequence regions are distinguished in MT1-MMP: Prodomain (Ser1-Arg88), catalytic domain (Tyr89-Gly261), junction between catalytic domain and hemopexin domain (Gly262-Gly292), hemopexin-like domain (Pro293-Cys485) and C-terminal sequence (Pro486-Val559) with transmembrane segment. A soluble form of MT1-MMP without transmembrane segment has been found in culture medium of a breast carcinoma cell line [Imai et al., 1996].

MT1-MMP is expressed in adult lung, placenta, kidney, ovaries, intestine, prostate and spleen [Will & Hinzmann, 1995]. Increased amounts of the enzyme are found in tumor tissues such as lung carcinoma [Butler et al., 1998], gastric carcinoma [Nomura et al., 1995], breast, head and neck carcinoma [Okada et al., 1995].

MT1-MMP is activated by removal of its prodomain. The reaction is catalyzed by furin, a subtilysin-type serine protease, which recognizes a motif of four basic amino acid residues located between the prodomain and catalytic domain [Pei & Weiss, 1996].

MT1-MMP activates progelatinase A [Sato et al., Strongin et al., 1995; Will et al., 1996] and procollagenase-3 [Knauper et al., 1996] by proteolytic cleavage of their domains. The ability of MT1-MMP to activate other matrix metalloproteinases provides potential for enhanced pericellular proteolysis in physiological and pathological processes. In particular, activation of progelatinase A by MT1-MMP is considered to contribute to local degradation of extracellular matrix during cell migration and proliferation. MT1-MMP also hydrolyzes fibrillar collagens I, II and III into characteristic ¾ and ¼ fragments [D′Ortho et al., 1997; Ohuci et al., 1997] and it cleaves a number of other ECM proteins, including fibronectin, vitronectin, laminin-1 and dermatan sulfate proteoglycan [D′Ortho et al., 1997; Pei & Weiss, 1996; Ohuci et al., 1997]. The activity of MT1-MMP is poorly inhibited by TIMP-1 but efficiently inhibited by TIMP-2 and TIMP-3 [Will et al., 1996].

CC1043 is a recombinant polypeptide sequence produced as a periplasmic protein in E. coli. The proenzyme consists of MT1-MMP residues corresponding to Ser1-Val501 followed by tone Thr-residue and six His-residues. The calculated Mr of the recombinant soluble proenzyme is 58200 Da.

Application

Useful as an antigen standard in immunoassays. The proenzyme can be activated with trace amounts of MT1-MMP catalytic domain (D′Ortho et al., 1997; Butler et al., 1998).

Physical form

Provided as a liquid in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl2.

Preparation Note

Maintain frozen at -70°C in undiluted aliquots. The enzyme may be stored at -20°C for several weeks. Repeated freezing and thawing should be avoided

Analysis Note

Appears as a predominant band at 58 kDa in SDS-PAGE

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1