CBL171

Anti-α-Smooth Muscle Actin (ACTA2) Antibody,

CHEMICON^®, mouse monoclonal, ASM-1

• Synonym(s): Alpha-actin-2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: ASM-1, monoclonal
• Species reactivity: chicken, mouse, horse, rat, human, bovine
• Application: IF, IHC (p), WB
• Citations: 53

Properties

• Product Name: Anti-Actin Antibody, smooth muscle, clone ASM-1, clone ASM-1, Chemicon^®, from mouse
• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: ASM-1, monoclonal
• species reactivity: chicken, mouse, horse, rat, human, bovine
• manufacturer/tradename: Chemicon^®
• technique(s): immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG2a
• NCBI accession no.: NP_001604
• UniProt accession no.: P62736
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... ACTA2(59)

Description

General description

43 kDa

ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. This antibody reacts with many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues, and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Myogenic soft tissues detected include leiomyosarcomas, leiomyomas, and certain stromal cells surrounding infiltrating ductal carcinoma of the breast.

Immunogen

Epitope: N-terminus

Synthetic peptide corresponding to the ten N-terminal amino acids of the alpha-smooth muscle isoform of actin.

Application

Anti-Actin Antibody, smooth muscle, clone ASM-1 detects level of Actin & has been published & validated for use in IF, IH, IH(P) & WB.

Immunohistochemistry:
1:2000 dilution of a previous lot was used on frozen and formalin-fixed, paraffin-embedded tissues; protease pretreatment is recommended for paraffin-embedded sections.

Immunofluorescence:
A previous lot of this antibody was used on Immunofluoresence.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Biochem/physiol Actions

Expected to cross-react with bovine, equine and chicken. Reactivity with other species has not been confirmed.

This antibody is specific for the alpha-smooth-muscle isoform of actin (MW 43 kDa).

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal IgG2a presented as lyophilized material. Reconstitute in 1 mL of of distilled water.
Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.

Preparation Note

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Positive reaction using stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.

Evaluated by Western Blot on HUVEC lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected ACTIN, SM MUSC on 10 μg of HUVEC lysates.

Other Notes

Replaces: 04-1040

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H312 + H332,H412
• pcodes: P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
• Storage Class: 11 - Combustible Solids