ABS234

Anti-PI3 Kinase Antibody, p85

from rabbit, purified by affinity chromatography

• Synonym(s): Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-al
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB
• Citations: 11

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology), monkey (based on 100% sequence homology), bovine (based on 100% sequence homology)
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_037137
• UniProt accession no.: Q63787
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: bovine ... Pik3R1(282307) ; human ... PIK3R1(5295) ; mouse ... Pik3R1(18708) ; rat ... Pik3R1(25513) ; rhesus monkey ... Pik3R1(698996)

Description

General description

Phosphatidylinositol 3-Kinase (PI3 Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β,γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibited by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.

~85 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to rat PI3 Kinase, p85.

Application

Detect PI3 Kinase, p85 using this rabbit polyclonal antibody, Anti-PI3 Kinase Antibody, p85 validated for use in western blotting & IP.

Immunoprecipitation Analysis: A representative lot immunoprecipitated PI3 Kinase, p85 in 0.5 mg of Jurkat cell lysate.

Analysis Note

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:600 dilution of this antibody detected PI3 Kinase, p85 in 10 µg of Jurkat cell lysate.

Other Notes

Replaces: 06-497

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable