ABF32

Anti-Fibroblast-specific Protein 1 (S100A4) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, WB
• Citations: 11

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, rat, human, horse
• species reactivity (predicted by homology): canine (based on 100% sequence homology), bovine (based on 100% sequence homology), equine (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_002952
• UniProt accession no.: P26447
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... S100A4(6275)

Description

General description

S100A4, also known as Fibroblast-specific Protein 1, is a member of the Ca2+- dependent S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 is a metastasis factor that may function in motility, invasion, and tubulin polymerization. Increased expression of S100A4 has been associated with various types of cancer.

~12 kDa observed

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human S100A4.

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected S100A4 in mouse neural stem cells.

Research Category
Stem Cell Research

Inflammation & Immunology

Research Sub Category
Ion & Transport Channels

Inflammation & Autoimmune Mechanisms

This Anti-Fibroblast-specific Protein 1 (S100A4) Antibody is validated for use in WB, IC for the detection of Fibroblast-specific Protein 1 (S100A4).

Biochem/physiol Actions

This antibody recognizes S100A4 at the C-terminus.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected S100A4 in 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable