ABC1691

Anti-PEPCK

from rabbit, purified by affinity chromatography

• Synonym(s): Phosphoenolpyruvate carboxykinase, cytosolic, PEPCK-C
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF, IHC, WB
• Citations: 1

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
• NCBI accession no.: NP_002582
• UniProt accession no.: P35558
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... PCK1(5105)

Description

General description

Phosphoenolpyruvate carboxykinase, cytosolic (UniProt: also known as PEPCK, PEPCK-C) is encoded by the PCK1 (also known as PEPCK1) gene (Gene ID: 5105) in human. PEPCK catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), which is a rate-limiting step in gluconeogenesis. It requires manganese as a cofactor. It is expressed in higher levels in liver of fasting subjects. Kidney and adipocytes exhibit lower expression levels. Two isozymes of PEPCK have been identified, cytosolic and mitochondrial. PEPCK consists of an N-terminal and a catalytic C-terminal domain, with the active site and metal ions located in a cleft between them. Upon binding to substrate, PEPCK undergoes a conformational change that accelerates the catalysis process. Expression of PEPCK can be controlled by cAMP, glucocorticoids, and by insulin. Its expression is induced by glucagon, catecholamines, and glucocorticoids during periods of fasting and in response to stress and is diminished following glucose-induced and by the action of insulin. PEPCK-C is absent in fetal liver but appears at birth, concomitant with the capacity for gluconeogenesis. Deficiency of PEPCK-C is a rare metabolic disorder results in fatty infiltration of liver and kidney, hypoglycemia, hepatomegaly, and failure to thrive.

~65 kDa observed; 69.19 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal half of human phosphoenolpyyruvate carboxykinase (PEPCK).

Application

Detect Phosphoenolpyruvate carboxykinase, cytosolic [GTP] using this rabbit polyclonal Anti-PEPCK, Cat. No. ABC1691, tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PEPCK in human kidney, human pancreas, human skeletal muscle, and human placental tissue.

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected PEPCK in human small intestine and differentiated duodenum intestinal organoid tissue sections.

Western Blotting Analysis: 2 µg/mL from a representative lot detected PEPCK in 10 µg of human kidney tissue.

Research Category
Apoptosis & Cancer

Biochem/physiol Actions

This polyclonal antibody detects cytosolic phosphoenolpyruvate carboxykinase in human kidney.

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunohistochemistry in human liver tissue

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PEPCK in human liver tissue.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable