AB5230

Anti-Calcium Channel Antibody, Voltage Gated β3

Chemicon^®, from rabbit

• Synonym(s): CAB3, Dihydropyridine-sensitive L-type, Calcium Channel beta-3 Subunit, Voltage-dependent Calcium Channel beta-3 Subunit
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 1

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• NCBI accession no.: NM_000725.2
• UniProt accession no.: P54284
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CACNB3(784)

Description

Immunogen

A purified peptide corresponding to amino acid residues 463-477 of rabbit Ca beta3 (Accession number P54286).

Epitope: Voltage Gated beta3

Application

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Signaling Neuroscience

This Anti-Calcium Channel Antibody, Voltage Gated β3 is validated for use in WB for the detection of Calcium Channel.

Western blot: 1:200-1:300 using ECL on rat brain membranes.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes the full length Ca beta3 protein, not with other known proteins tested so far.

Physical form

Format: Purified

Purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Preparation Note

Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3