AB3375

Anti-NFκB p65 Antibody, phospho-specific (Ser276)

serum, Chemicon^®

• Synonym(s): Anti-Anti-CMCU, Anti-Anti-NFKB3, Anti-Anti-p65
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human
• Application: ELISA, WB
• Citations: 4

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, western blot: suitable
• NCBI accession no.: NM_021975.2
• UniProt accession no.: Q04206
• shipped in: wet ice
• target post-translational modification: phosphorylation (pSer276)
• Gene Information: human ... NFKB1(4790)

Description

Immunogen

Epitope: phospho-specific (Ser276)

Synthetic peptide from human NFkappaB p65, phosphoSer276.

Application

Anti-NFκB p65 Antibody, phospho-specific (Ser276) is a high quality Rabbit Polyclonal Antibody for the detection of NFκB p65 & has been validated in ELISA & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western blot: 1:1,000. A 65 kDa band is observed against human p65. HeLa cells of TNF inducible KBM-5 cells can be used as a positive control.

ELISA: 1:10,000-1:30,000

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

NFkappaB p65, phosphoSer276. By Western blot the antibody shows minimal reactivity with non-phosphorylated p65 and by ELISA is shows minimal reactivity with the non-phosphorylated form of the immunogen peptide.

Physical form

Rabbit serum. Liquid containing 0.1% sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1