AB3279

Anti-Hexokinase Type II Antibody

Chemicon^®, from rabbit

• Synonym(s): HK II
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA, ICC, WB
• Citations: 11

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_000189.4
• UniProt accession no.: P52789
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... HK2(3099)

Description

General description

102 kDa

Immunogen

Recombinant rat Type II hexokinase.

Application

Anti-Hexokinase Type II Antibody is an antibody against Hexokinase Type II for use in ELISA, IC & WB.

Research Category
Metabolism

Research Sub Category
Enzymes & Biochemistry

Western Blot: 1:5,000-1:10,000.

Immunocytochemistry using indirect immunofluorescence.

ELISA

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Reacts with the Type II isozyme of rat hexokinase (102 kDa). No reactivity with the Type I or Type III isozymes of rat hexokinase by immunoblot or ELISA. Reactivity with human and hamster Type II isozyme has been confirmed by immunoblotting, and reactivity with Type II isozyme from other mammalian species is expected.

Physical form

Format: Purified

Immunodepleted Serum

Liquid. Partially Purified rabbit Serum. Antibodies reactive with the Type I isozyme were present in the original antiSerum; these were removed by affinity chromatography on a column of immobilized Type I isozyme, and the affinity-depleted Serum restored to original volume.

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
insulin-responsive tissues such as skeletal muscle

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids