AB3184

Anti-MEK5 Antibody

Chemicon^®, from rabbit

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA, IHC, IP, WB
• Citations: 6

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: Xenopus, canine, rabbit, sheep, monkey, human, mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NM_002757.2, NM_145160.1
• UniProt accession no.: Q13163
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MAP2K5(5607) ; mouse ... Map2K5(23938)

Description

Immunogen

Human MEK 5 synthetic peptide (aa. 59-74; Genbank Accession # U71088), conjugated to KLH; identical to rat MEK5 sequence over these residues.

Application

Anti-MEK5 Antibody is an antibody against MEK5 for use in ELISA, IH, IP & WB.

Research Category
Signaling

Research Sub Category
MAP Kinases

Western Blot: 1 μg/mL

Immunohistochemistry: 1:1,000

Immunoprecipitation

EIA

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

MEK 5 is also known as MAP Kinase Kinase 5, or ERK Kinase 5. AB3184 detects a 49 kDa protein, corresponding to the expeted molecular mass of Mek5, in rat brain, rat liver, rat kidney, rat heart, mouse brain, and mouse testis tissue extracts as well as in cell lysates prepared from the rat pheochromacytoma (PC12) and the human HeLa cell lines. No cross-reactivity with MEK 1, 2, 3, 4 or 6 has been observed with this antibody.

Physical form

Affinity purified immunoglobulin. Affinity purified immunoglobulin

Liquid containing PBS, pH 7.2. No preservatives.

Preparation Note

Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Thawed product may be stored at 2-8°C for up to 4 weeks. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable