AB1529

Anti-Nitric Oxide Synthase I Antibody

serum, Chemicon^®

• Synonym(s): NOS I, bNOS, nNOS
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 45

Properties

• biological source: sheep
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rabbit, rat, guinea pig
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_000620.1
• UniProt accession no.: P29475
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: guinea pig ... Nos1(100729682) ; rabbit ... Nos1(100009243) ; rat ... Nos1(24598)

Description

Immunogen

A 21 amino acid peptide (1409-1429) of the neuronal form of NOS from rat cerebellum, coupled to KLH. The sequence is H-RSESIAFIEESKKDADEVFSS-NH2.

Application

Detect Nitric Oxide Synthase I using this Anti-Nitric Oxide Synthase I Antibody validated for use in WB, IH.

Immunohistochemistry: 1:500-1,000

Immunoblotting

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

Biochem/physiol Actions

bNOS (neuronal). Not yet tested for cross-reactivity to iNOS or eNOS. See reference below.

Physical form

Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue.

Preparation Note

Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable