539251

Protein A, Alkaline Phosphatase Conjugate

This Protein A, Alkaline Phosphatase Conjugate is validated for use in Direct Immunoassay.

• NACRES: NA.41
• UNSPSC Code: 41116133
• Form: liquid
• Biological source: Staphylococcus aureus

Properties

• biological source: Staphylococcus aureus
• Quality Segment: 100
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Direct Immunoassay (1:5000))
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Native protein A conjugated to alkaline phosphatase. Can be used instead of an alkaline phosphatase-conjugated secondary antibody in many immunological assays.

Native protein A from Staphylococcus aureus conjugated to alkaline phosphatase.

Application

Direct Immunoassay (1:5000)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM Tris, 1 mM MgCl₂, 1% BSA, pH 8.0.

Analysis Note

Single band by SDS-PAGE

Other Notes

p-Nitrophenyl phosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl₂ at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable