524751-M

Phosphodiesterase 7A1, Human, Recombinant, S. frugiperda

• Synonym(s): hPDE7A1
• UNSPSC Code: 12352203
• NACRES: NA.77
• Specific activity: ≥0.5 U/μL
• Recombinant: expressed in baculovirus infected Sf21 cells

Properties

• recombinant: expressed in baculovirus infected Sf21 cells
• Quality Level: 100
• form: liquid
• specific activity: ≥0.5 U/μL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• storage temp.: −70°C

Description

General description

Full-length, recombinant, human phosphodiesterdase 7A1 (PDE7A1) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE7A1 is required for T-cell activation induced by the costimulation of CD3 and CD28 receptors. Increased PDE7A1 correlates with a decrease in cAMP, increased IL-2, and increased cell proliferation. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.

Physical form

In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Han, P., et al. 2006. J. Biol. Chem.281, 15050.
Lugnier, C. 2006. Pharmacol Ther.109, 366.
Bloom, T.J., and Beavo, J.A. 1996. Proc. Natl. Acad. Sci. U S A93, 14188.

One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• signalword: Danger
• hcodes: EUH430
• pcodes: P201 - P202 - P273 - P391 - P405 - P501
• Hazard Classifications: ED ENV 1