475941

Myokinase, Yeast

• Synonym(s): Myokinase, Yeast, Adenylate Kinase, ATP:AMP Phosphotransferase
• CAS Number: 9013-02-9
• EC Number: 2.7.4.3 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.54
• MDL number: MFCD00131644
• eCl@ss: 32160410
• Specific activity: ≥10 units/mg solid, ≥200 U/mg

Properties

• form: lyophilized
• Quality Segment: 100
• specific activity: ≥10 units/mg solid, ≥200 U/mg
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic)
• pI: 5.7
• solubility: water: 1 mg/mL
• foreign activity: ATPase ≤0.01%, Phosphoglycerate kinase ≤0.1%
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Native, yeast myokinase. Myokinase reversibly transfers phosphate from ATP to AMP, thus forming two molecules of ADP. The enzyme is specific for adenine nucleotides and catalyzes the reaction only in the presence of a divalent metal ion (e.g. Mg²⁺, Ca²⁺, Co²⁺, Mn²⁺, or Ni²⁺).

Note: 1 KU = 1000 units.

Physical form

Lyophilized from potassium phosphate buffer.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Makarchikov, A.F., et al. 2002. Biochem. Biophys. Acta.1592, 117.
Schricker, R., et al. 2002. J. Biol. Chem.277, 28757.
Magdolen, V., et al. 1987. Curr. Genet.12, 405.
Tomasselli, A.G., et al. 1986. Eur. J. Biochem.155, 111.
Ito, Y., et al. 1980. Eur. J. Biochem.105, 85.

One unit is defined as the amount of enzyme that will convert 1.0 µmol ATP and 1.0 µmol AMP to 2 µmol ADP per min at 25°C, pH 7.5. Note: 1 KU = 1000 units.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable