This product is lyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.
Our recommendation is to further dilute in 100 mM NaCl, 50 mM sodium acetate, pH 6.0. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H?O.
https://b2b.sigmaaldrich.com/US/en/product/mm/475911#product-documentation
475911
Myeloperoxidase, Human Polymorphonuclear Leukocytes
A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins.
Synonym(s):
Myeloperoxidase, Human Polymorphonuclear Leukocytes, MPO
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.25
Specific activity:
150-200 units/mg protein
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Pricing and availability is not currently available.
biological source
human
Quality Segment
assay
≥95% (SDS-PAGE)
form
lyophilized
specific activity
150-200 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
water: soluble (volume is lot-specific and can be found on the product label)
shipped in
wet ice
storage temp.
−20°C
SMILES string
[O+H2]O[O-]
InChI
1S/H2O3/c1-3-2/h1-2H
InChI key
JSPLKZUTYZBBKA-UHFFFAOYSA-N
General description
Native, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins.
Physical form
Lyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 1 year at -20°C or -70°C.
Further dilute in 100 mM NaCl, 50 mM sodium acetate, pH 6.0. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H₂O.
Other Notes
Nauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol.107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun.189, 1129.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol.107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun.189, 1129.
One unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
|---|---|---|---|
| specific activity 150-200 units/mg protein | specific activity ~150 U/mg | specific activity ≥250 units/mg solid (using pyrogallol) | specific activity ≥250 units/mg solid (using pyrogallol) |
| assay ≥95% (SDS-PAGE) | assay - | assay - | assay - |
| biological source human | biological source horseradish | biological source - | biological source - |
| form lyophilized | form powder | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder |
| storage condition OK to freeze | storage condition - | storage condition - | storage condition - |
| solubility water: soluble (volume is lot-specific and can be found on the product label) | solubility 50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA) | solubility 0.1 M phosphate buffer: soluble (pH 6.0), H2O: soluble | solubility 0.1 M phosphate buffer: soluble 10 mg/mL, clear, orange to red (pH 6.0) |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Dear Tecnical Support, How should I reconstitute this product?Would it be fine that I reconstitute it by adding 100 microliter of deionized water, so that I obtain a 1mg/mL of protein in the original buffer? Then further dilute it in the indicated buffer?
1 answer-
Helpful?
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