444232

MMP-9, Dimer, Human Neutrophil

• Synonym(s): Matrix Metalloproteinase 9, Gelatinase B, 92 kDa type IV Collagenase
• EC Number: 3.4.24.35 (BRENDA, IUBMB)
• NACRES: NA.77
• UNSPSC Code: 12352202
• Specific activity: ≥1000 mU/mg protein
• Assay: ≥90% (SDS-PAGE)

Properties

• assay: ≥90% (SDS-PAGE)
• Quality Segment: 100
• form: liquid
• specific activity: ≥1000 mU/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• foreign activity: other MMP activity, none detected
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Dimeric, native MMP-9 from stimulated human neutrophils. Enzyme should be activated just prior to use.

M.W. 222,000 (non-reducing SDS-PAGE). Note: 1 mU = 1 milliunit.

Physical form

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% 35 Detergent, 0.05% NaN₃, pH 7.0.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Other Notes

Olson, M.W. et al. 2000. J. Biol. Chem.275, 2661.
Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable