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About This Item
NACRES:
NA.74
UNSPSC Code:
12352203
Clone:
HP6069, monoclonal
Species reactivity:
-
Application:
—
Citations:
4
Pricing and availability is not currently available.
biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
secondary antibodies
clone
HP6069, monoclonal
form
liquid
does not contain
preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
General description
Purified mouse monoclonal antibody. Recognizes human IgG1 Fc fragment.
This Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) is validated for use in ELISA for the detection of Human IgG₁, Fc Fragment Specific.
Application
ELISA (see comments and application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, pH 7.4.
Preparation Note
Following initial use aliquot and freeze (-20°C).
Other Notes
Can be used as a capture antibody for ELISA at 10 µg/ml in PBS without carrier protein. Antibody should be titrated for optimal results in individual systems.
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 0.1%
Human IgG3: 0.1%
Human IgG4: <0.02%
Human IgA: <0.1%
Human IgE: <0.1%
Human IgM: <0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: <0.01%
Human IgG2: <0.01%
Human IgG3: <0.01%
Human IgG4: <0.01%
Human IgA: <0.01%
Human IgE: <0.001%
Human IgM: <0.01%
Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: 100%
Human IgG2: 0.1%
Human IgG3: 0.1%
Human IgG4: <0.02%
Human IgA: <0.1%
Human IgE: <0.1%
Human IgM: <0.1%
Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: <0.01%
Human IgG2: <0.01%
Human IgG3: <0.01%
Human IgG4: <0.01%
Human IgA: <0.01%
Human IgE: <0.001%
Human IgM: <0.01%
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
1 of 1
This Item | |||
|---|---|---|---|
| biological source mouse | biological source mouse | biological source goat | biological source goat |
| antibody form purified antibody | antibody form purified antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| clone HP6069, monoclonal | clone HP6043, monoclonal | clone polyclonal | clone polyclonal |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| isotype IgG1 | isotype IgG | isotype - | isotype - |
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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