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391340
HEPES
≥99% (alkalimetric), solid, pH 6.8-8.2, Molecular Biology
Synonym(s):
HEPES, N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
About This Item
Product Name
HEPES, Free Acid, Molecular Biology Grade, A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.
grade
Molecular Biology
Quality Segment
description
Merck USA index - 14, 4654
assay
≥99% (alkalimetric)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
useful pH range
6.8-8.2
pKa (25 °C)
7.5
solubility
water: 400 mg/mL, aqueous base: soluble
cation traces
heavy metals: ≤0.0005%
foreign activity
DNase, RNase, and protease, none detected
shipped in
ambient
storage temp.
15-25°C
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
General description
Application
- Fluorescent labeling of the root cap cells with the bioactive NBD-S chemical probe based on the cellulose biosynthesis inhibition herbicides: This innovative study demonstrates the application of molecular biology grade HEPES in the formulation of bioactive probes for the fluorescent labeling of root cap cells. Using cellulose biosynthesis inhibition herbicides as a model system, this research provides valuable insights into plant biology, aiding in the understanding of plant cell wall structures and functions. The use of HEPES helps maintain the physiological pH during the experiments, ensuring the stability and effectiveness of the chemical probes used (Tamogami S et al., 2021).
Legal Information
Disclaimer
1 of 1
This Item | |||
|---|---|---|---|
| assay ≥99% (alkalimetric) | assay 0.9—1.1 M | assay 0.9—1.1 M | assay - |
| grade Molecular Biology | grade - | grade - | grade BioPerformance Certified |
| Quality Level 100 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| form solid | form liquid | form liquid | form liquid |
| storage condition OK to freeze | storage condition (Tightly closed. Dry), dry at room temperature | storage condition (Tightly closed. Dry), dry at room temperature | storage condition - |
| storage temp. 15-25°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
| assay ≥99% (alkalimetric) | assay 0.9—1.1 M | assay 0.9—1.1 M | assay - |
| grade Molecular Biology | grade - | grade - | grade BioPerformance Certified |
| Quality Level 100 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| form solid | form liquid | form liquid | form liquid |
| storage condition OK to freeze | storage condition (Tightly closed. Dry), dry at room temperature | storage condition (Tightly closed. Dry), dry at room temperature | storage condition - |
| storage temp. 15-25°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
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Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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