374807

Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg²⁺ Buffer

Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg2+ buffer, CAS 9013-72-3, is a large, multi-subunit, O2-carrying, metalloprotein that is used in the production of antibodies.

• Synonym(s): Hemocyanin, Keyhole Limpet, Megathura crenulata, BES/Mg²⁺ Buffer, KLH
• CAS Number: 9013-72-3
• UNSPSC Code: 12352202
• NACRES: NA.41
• MDL number: MFCD00131280

Properties

• biological source: Megathura crenulata
• Quality Segment: 100
• assay: ≥90% (size exclusion chromatography)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• color: clear blue-gray
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Keyhole limpet hemocyanin (KLH) is a large, multi-subunit, O2-carrying, metalloprotein. It is isolated from a marine snail species called Megathura crenulate.[1]

Biochem/physiol Actions

Keyhole limpet hemocyanin (KLH) exhibits immunostimulatory properties and stimulates both cell-mediated and humoral responses in animals and humans.[1] It serves as a vaccine adjuvant[1] and facilitates protein conjugation. In addition, KLH acts as a carrier protein to produce antibodies as well as haptens such as peptides and oligosaccharides. It is also used in the treatment of bladder cancer.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM MgSO₄, 5 mM BES buffer, 0.9% NaCl, pH 6.5. Sterile-filtered.

Preparation Note

KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2