344146
FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli
FPTase, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, is composed of an74 kDa α- and 47 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence.
Synonym(s):
FTase, Farnesyl Proteintransferase
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.54
Recommended Products
recombinant
expressed in E. coli
Quality Level
Assay
≥90% (SDS-PAGE)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity.
Recombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion protein with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity.
Biochem/physiol Actions
1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.
Warning
Toxicity: Standard Handling (A)
Physical form
In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Zimmerman, K.K., et al. 1998. Protein Expr. Purif.14, 395.
Reiss, Y., et al. 1990. Cell62, 81.
Reiss, Y., et al. 1990. Cell62, 81.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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