This product has not been tested for suitability on Yarrowia lipolytica. However, the reagent has been used successfully by other researchers. Please see the link below to review a publication that may be helpful:
https://pmc.ncbi.nlm.nih.gov/articles/PMC4955270/
71186
YeastBuster Protein Extraction Reagent
Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis
Synonym(s):
Yeast protein extraction reagent
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About This Item
NACRES:
NA.77
UNSPSC Code:
41106512
Pricing and availability is not currently available.
form
liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze
shipped in
wet ice
General description
Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis
YeastBuster<TMSYMBOL></TMSYMBOL> Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The reagent avoids harsh conditions of vigorous mechanical or chemical treatment that often result in heat degradation of target proteins. The proprietary formulation utilizes a mix of mild detergent, protein stabilization buffer, and tris(hydroxypropyl) phosphine (THP) reducing agent (THP concentrate provided separately). This powerful combination eliminates the inconsistencies associated with tedious mechanical disruption of yeast cells with glass bead abrasives, ultrasonication and pressure disruption, or enzymatic digestion with β-1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells.
In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind<TMSYMBOL></TMSYMBOL> and Ni-NTA HIS-BIND® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.
In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind<TMSYMBOL></TMSYMBOL> and Ni-NTA HIS-BIND® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.
Features and Benefits
- Gentle, rapid, efficient extraction of proteins from yeast cells
- Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
- Higher yield of total and enzymatically active proteins
- Fully compatible with Ni-NTA His•Bind and GST•Bind affinity purification methods
Other Notes
•100 ml or 500 mlYeastBuster Protein Extraction Reagent
•1 ml or 5 ml100X THP Solution
•1 ml or 5 ml100X THP Solution
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Legal Information
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
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This Item | |||
|---|---|---|---|
| storage condition OK to freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles |
| form liquid | form liquid | form liquid | form liquid |
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® |
| shipped in wet ice | shipped in - | shipped in - | shipped in wet ice |
signalword
Warning
hcodes
pcodes
Hazard Classifications
STOT RE 2 Oral
target_organs
Kidney
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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I want to use it for lysis of Yarrowia lipolytica. Is this going to work for the same?
1 answer-
Helpful?
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Does YeastBuster denature the enzymes that are liberated from yeast? Or generally do most intracellular enzymes retain activity? Thanks.
1 answer-
This product is a proprietary formulation that utilizes a mix of mild detergents and protein stabilization buffer that, when combined with tris(hydroxypropyl)phosphine (THP)
reducing agent, resulting in higher total protein yields the recovery of enzymatically active proteins.Helpful?
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Cat #71186 YeastBuster Protein Extraction Reagent inquiry: What are the ingredients of this reagent? Is there a detergent in the reagent? I understand if the actual concentrations are proprietary, but I would appreciate knowing what the ingredients are.
1 answer-
As provided, this proprietary formulation containing ethylene glycol, lithium chloride and Tris(hydroxypropyl)phosphine is in a mixture with mild zwitterionic detergent.
Helpful?
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