324740

Elastase Substrate V, Fluorogenic

≥95% (HPLC), powder, lyophilized solid, elastase substrate, Calbiochem^®

• Synonym(s): Elastase Substrate V, Fluorogenic, MeOSuc-AAPV-AMC
• Empirical Formula (Hill Notation): C₃₁H₄₁N₅O₉
• CAS Number: 72252-90-5
• Molecular Weight: 627.69
• UNSPSC Code: 12352200
• NACRES: NA.25
• MDL number: MFCD00077135
• Assay: ≥95% (HPLC), ≥95% (TLC)
• Form: lyophilized solid, powder
• Storage condition: OK to freeze, desiccated, protect from light

Properties

• Product Name: Elastase Substrate V, Fluorogenic, Elastase Substrate V, Fluorogenic, CAS 72252-90-5, is a sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM).
• Quality Segment: 200
• assay: ≥95% (HPLC), ≥95% (TLC)
• form: lyophilized solid, powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated, protect from light
• color: white to off-white
• solubility: ethanol: 1 mg/mL, DMF: 5 mg/mL, DMSO: 5 mg/mL
• fluorescence: λ_ex 360-380 nm, λ_em 430-460 nm
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: N1([C@@H](CCC1)C(=O)NC(=O)[C@@H](Nc2cc3c(cc2)C(=CC(=O)O3)C)C(C)C)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)CCC(=O)OC)C)C
• InChI: 1S/C31H41N5O9/c1-16(2)27(34-20-9-10-21-17(3)14-26(39)45-23(21)15-20)30(42)35-29(41)22-8-7-13-36(22)31(43)19(5)33-28(40)18(4)32-24(37)11-12-25(38)44-6/h9-10,14-16,18-19,22,27,34H,7-8,11-13H2,1-6H3,(H,32,37)(H,33,40)(H,35,41,42)/t18-,19-,22-,27-/m0/s1
• InChI key: NHMQNKPYBPDCAY-NFHWZJRKSA-N

Description

General description

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte (K_m = 140 µM; K_cat/K_m = 120,000 s^-1 M^-1) and porcine pancreatic elastase. Not hydrolyzed by cathepsin G. Excitation max.: ~380 nm; emission max.: ~460 nm.

Biochem/physiol Actions

Cell permeable: no

Km = 140 µM; kcat/Km = 120,000 M⁻¹ s⁻¹as substrates for human leukocyte and porcine pancreatic elastase

Primary Target
Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase

Product does not compete with ATP.

Reversible: no

Preparation Note

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.

Other Notes

Barrett, A.J. 1981. Methods Enzymol. 80, 581.
Castillo, M.J., et al. 1979. Anal. Biochem.99, 53.
Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.

MeOSuc-Ala-Ala-Pro-Val-AMC

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable