324708

Asp-N Protease

Cleaves N-terminal to aspartic acid residues, suitable for Mass Spectrometry, from Pseudomonas fragi mutant strain

• Synonym(s): Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi
• CAS Number: 9001-92-7
• EC Number: 3.4.24.33 (BRENDA, IUBMB)
• MDL number: MFCD00004464
• NACRES: NA.54
• UNSPSC Code: 12352202
• eCl@ss: 32160410
• Specific activity: ≥20,000 units/mg protein
• Assay: ≥90% (SDS-PAGE)

Properties

• Product Name: Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi,
• grade: Proteomics Grade
• Quality Segment: 100
• assay: ≥90% (SDS-PAGE)
• form: lyophilized solid
• specific activity: ≥20,000 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: water: soluble
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Physical form

Lyophilized from 10 mM Tris-HCl, pH 7.5.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Other Notes

Drapeau, G.R. 1980. J. Biol. Chem.255, 839.

One unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1