We have not studied product 234167 in denatured conditions for a Western blot. Here are some literature references one can browse through: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C22&q=234167+western+blot+sigma&btnG=
234167
Anti-Collagen Type I Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-Collagen I Antibody
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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
mouse, human, rat
Application:
—
Citations:
48
biological source
rabbit
Quality Segment
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
mouse, human, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(Frozen Sections (1:30-1:60)
Immunoblotting (1:200-1:500)
Immunofluorescence (1:20-1:40)
Immunoprecipitation (1:20-1:60))
isotype
IgG
shipped in
wet ice
storage temp.
−70°C
target post-translational modification
unmodified
General description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~115 kDa (doublet) type I collagen protein.
Recognizes type I collagen. Exhibits slight cross-reactivity with type III collagen.
This Anti-Collagen Type I Rabbit pAb is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of Collagen Type I.
Immunogen
Fetal Mouse Skin
purified, fetal mouse skin collagen type I
Application
Frozen Sections (1:30-1:60, see comments)
Immunoblotting (1:200-1:500)
Immunofluorescence (1:20-1:40)
Immunoprecipitation (1:20-1:60)
Immunoblotting (1:200-1:500)
Immunofluorescence (1:20-1:40)
Immunoprecipitation (1:20-1:60)
Physical form
Undiluted serum.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Romanos, G.E., et al. 1992. J. Periodont. Res. 27, 101.
Zimmermann, B., et al. 1992. Eur. Arch. Biol. 103, 93.
Schröter-Kermani, C., et al. 1991. Matrix 11, 428.
Zimmermann, B., et al. 1992. Eur. Arch. Biol. 103, 93.
Schröter-Kermani, C., et al. 1991. Matrix 11, 428.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
1 of 1
This Item | |||
|---|---|---|---|
| biological source rabbit | biological source rabbit | biological source rabbit | biological source mouse |
| antibody form serum | antibody form serum | antibody form serum | antibody form purified antibody |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| species reactivity mouse, human, rat | species reactivity human, rat, mouse | species reactivity human, mouse, rat | species reactivity rat, human, bovine, rabbit |
| form liquid | form liquid | form liquid | form liquid |
| clone polyclonal | clone polyclonal | clone polyclonal | clone II-4C11, monoclonal |
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Hi, Does this col I antibody 234167 work in denatured conditions in a western blot?
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