16-157

Protein A Agarose/Salmon Sperm DNA, 2.5 mL

for use in chromatin immunoprecipitations (ChIP assays)

• Synonym(s): ChIP agarose beads, ChIP agaraose A beads, ChIP assays
• UNSPSC Code: 12352202
• NACRES: NA.32
• eCl@ss: 32160405
• Form: liquid
• Biological source: Staphylococcus aureus

Properties

• biological source: Staphylococcus aureus
• Quality Segment: 100
• form: liquid
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable
• suitability: suitable for immunoprecipitation
• shipped in: wet ice

Description

General description

Protein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.

Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.

Application

Suitable for use in chromatin immunoprecipitation (ChIPs) assays

Physical form

10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.

Analysis Note

Routinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1