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Chromolith® WP 300 Protein A (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

Synonym(s):

Chromolith® WP 300 Protein A 25-4.6 HPLC column

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1 unit
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CZK 36,400.00

About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
Matrix active group:
Protein A bonding phase
Pore size:
2 μm macroporosity, 30 nm mesoporosity
Matrix:
monolithic silica

CZK 36,400.00


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Product Name

Chromolith® WP 300 Protein A, L × I.D. 25 mm x 4.6 mm HPLC column

material

PEEK column

product line

Chromolith®

feature

endcapped: no

packaging

1 ea of

parameter

2 - 45 °C temp. range, 2-45 °C temp. range, 200 bar pressure

technique(s)

HPLC: suitable, LC/MS: suitable

column L × I.D.

2.5 cm × 4.6 mm

surface area

120 m2/g

matrix

monolithic silica

matrix active group

Protein A bonding phase

pore size

2 μm macroporosity, 30 nm mesoporosity

operating pH range

1.5-7.5

operating pH

1.5-7.5

separation technique

affinity

storage temp.

2-8°C

General description

Affinity chromatography is a selective technique which takes advantage of specific molecular interactions, for example antigen and antibody. The Chromolith® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Chromolith® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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1.523581.522701.52265
matrix active group

Protein A bonding phase

matrix active group

Protein A bonding phase

matrix active group

C18 bonding phase

matrix active group

C8 bonding phase

separation technique

affinity

separation technique

-

separation technique

reversed phase

separation technique

reversed phase

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

product line

Chromolith®

product line

-

product line

Chromolith®

product line

Chromolith®

pore size

2 μm macroporosity, 30 nm mesoporosity

pore size

2 μm macroporosity, 30 nm mesoporosity

pore size

2 μm macroporosity, 30 mm mesoporosity, 30 nm mesoporosity

pore size

2 μm macroporosity, 30 nm mesoporosity

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)


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Certificates of Analysis (COA)

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Articles

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

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HPLC separuje a identifikuje velké biomolekuly, jako jsou proteiny a peptidy, přečerpáváním vzorku přes kolonu naplněnou sorbentem.

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

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Global Trade Item Number

SKUGTIN
152258000104054839085284

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