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1.52252

Chromolith® WP 300 Epoxy (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

Synonym(s):

Chromolith® WP 300 Epoxy 25-4.6 HPLC column

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1 unit
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CZK 16,200.00

About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
Matrix active group:
Epoxy bonding phase
Pore size:
2 μm macroporosity, 30 nm mesoporosity
Matrix:
monolithic silica

CZK 16,200.00


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Product Name

Chromolith® WP 300 Epoxy, L × I.D. 25 mm x 4.6 mm HPLC column

material

PEEK column

Quality Level

product line

Chromolith®

feature

endcapped: no

packaging

1 ea of

extent of labeling

5-6% Carbon loading

parameter

2 - 60 °C temp. range, 2-60 °C temp. range, 200 bar pressure

technique(s)

HPLC: suitable, LC/MS: suitable

column L × I.D.

2.5 cm × 4.6 mm

surface area

120 m2/g

matrix

monolithic silica

matrix active group

Epoxy bonding phase

pore size

2 μm macroporosity, 30 nm mesoporosity

operating pH range

1.5-7.5

storage temp.

no temp limit

General description

Chromolith® WP 300 Epoxy columns are specially designed for the user-specific immobilization of ligands and their later application in HPLC. The unique bimodal pore structure of silica monoliths allows efficient coupling independent of molecule size. The wider mesopores also enable the use of proteins and antibodies as both ligand immobilized on the column, and later analyte separated by an immobilized column.
Potential applications: attach trypsin to obtain HPLC column-protein digestion reactor; attach protein and measure other protein interaction with the attached one; attach any chiral selector to obtain a chiral column; attach any affinity ligand to obtain custom made affinity column, etc.

Chromolith® WP 300 Epoxy 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Easy to perform immobilization of ligand opens up limitless customization options
—Monolithic format allows for high flow rates at low backpressure, enabling high throughput

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Chromolith® WP 300 Epoxy is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Create Your Own Column Chemistry - Chromolith® WP 300 Epoxy 2 mm I.D. HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
1.522511.522501.52351
matrix active group

Epoxy bonding phase

matrix active group

Epoxy bonding phase

matrix active group

Epoxy bonding phase

matrix active group

Epoxy bonding phase

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable

pore size

2 μm macroporosity, 30 nm mesoporosity

pore size

2 μm macroporosity, 30 nm mesoporosity

pore size

2 μm macroporosity, 30 nm mesoporosity

pore size

2 μm macroporosity, 30 nm mesoporosity

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

material

PEEK column

material

PEEK column

material

PEEK column

material

-


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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Related Content

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy





Global Trade Item Number

SKUGTIN
152252000104054839085253

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