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14-392

Green Fluorescent Protein

Synonym(s):

GFP

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Size/SKUAvailabilityPrice
300 μg

Available to ship TODAYfromAreál Kühne+Nagel spol. s r.o.

CZK 11,200.00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
eCl@ss:
32160405
Form:
liquid
Assay:
>70%
Recombinant:
expressed in E. coli
Mol wt:
Mw ~28 kDa

CZK 11,200.00

Available to ship TODAYDetails

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recombinant

expressed in E. coli

Quality Segment

100

assay

>70%

form

liquid

mol wt

Mw ~28 kDa

packaging

pkg of 300 μg

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

GenBank accession no.

AAA27722

UniProt accession no.

P42212

shipped in

dry ice

General description

N-terminal HIS-tagged fusion protein corresponding to full-length Green Fluorescent Protein, expressed in E. coli. Green fluorescent protein (GFP) is a cytoplasmic protein found in jellyfish Aequorea Victoria.[1]

Biochem/physiol Actions

Green fluorescent protein (GFP) is genetically fused to many proteins in various species to generate non-reactive chimeras which are known to preserve their original biological activity as well as the fluorescent properties of native GFP. GFP acts as a reporter for gene expression and as a fusion tag for protein localization studies in live cells.[1] It serves as a spectroscopic and microscopic probe to understand the biological functions of various systems.[2] GFP is highly stable and can serve as a tool for in vitro studies.[1]

Physical form

PBS containing 20% glycerol.

Preparation Note

1 year at -20°C

Analysis Note

Routinely evaluated as a standard on protein gels and western immunoblots, for calibration of fluorometers, and microinjection into cells and tissues.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, immunocytochemistry: suitable, western blot: suitable

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

assay

>70%

assay

-

assay

-

assay

-

mol wt

Mw ~28 kDa

mol wt

-

mol wt

-

mol wt

-

form

liquid

form

-

form

-

form

-

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

  1. このGFPはwild typeのGFPでしょうか。それともEGFPなど他の種類のGFPでしょうか。

    1 answer

    1. eGFP stands for enhanced green fluorescent protein, a red-shifted mutant of GFP, which folds more efficiently and fluoresces more intensely than wild-type GFP. This product corresponds to the green fluorescent protein (GFP) isolated from the jellyfish, Aequorea victoria.
      Please also see: https://www.ncbi.nlm.nih.gov/protein/AAA27722 and https://www.uniprot.org/uniprot/P42212.

      GFP absorbs blue light (maximum 395 nm, smaller absorbance peak at 470 nm) and emits green light (maximum 507-510 nm, shoulder at 545 nm) (PubMed:8137953, PubMed:9154981). The exact value of the emission maximum depends on the environment of the chromophore (PubMed:10220315).

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