06-896

Anti-GFP (Green Fluorescent Protein) Antibody

Upstate^®, from chicken

• Synonym(s): Green fluorescent protein
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, WB
• Citations: 56

Properties

• biological source: chicken
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: vertebrates
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgY
• UniProt accession no.: P42212
• shipped in: dry ice
• target post-translational modification: unmodified

Description

General description

30 kDa

Green fluorescent protein (GFP) is a 29 kDa fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488 nm), GFP strongly fluoresces (Emission max: 507 nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells.

Immunogen

His-tagged green fluorescent protein of Aequorea victoria.

Application

Research Sub Category
Epitope Tags

Research Category
Epitope Tags & General Use

Anti-GFP (Green Fluorescent Protein) Antibody detects level of GFP (Green Fluorescent Protein) & has been published & validated for use in IC & WB.

Immunocytochemistry:
This antibody was reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein.

Biochem/physiol Actions

Recognizes green fluorescent protein (GFP), Mr 30 kDa, and GFP-fusion proteins.

Physical form

Ammonium Sulfate Precipitation and PEG

Format: Purified

Purified chicken polyclonal IgY from egg yolk in buffer containing 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Analysis Note

Control
Rat brain lysate, cells expressing GFP protein.

Routinely evaluated by Western Blot on rat brain lysate containing purified GFP (Catalog #14-392) or a GFP-fusion protein from a transfected E. coli cell lysate.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected Green Fluorescent Protein from 50 μg of rat brain lysate containing 25 ng of purified Green Fluorescent Protein (Catalog # 14-392). A previous lot of this antibody also detected a GFP-fusion protein from a transfected E. coli cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1