12-500

HeLa + EGF Stimulated Cell Lysate in Mg²⁺ Lysis Buffer

• UNSPSC Code: 41105330
• NACRES: NA.42
• eCl@ss: 32011807

Properties

• biological source: human
• Quality Segment: 100
• form: liquid
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• shipped in: dry ice

Description

General description

EGF stimulated HeLa cell lysate. The cell lysate was quantitated via BCA assay. This sell lysate demonstrated higher levels of Ras-GTP form present as compared to unstimulated HeLa cell lysates. The assay was performed using the Ras GTPase Activation ELISA Kit (Catalog # 17-424)

Biochem/physiol Actions

HeLa

Physical form

100 &micor;g of EGF stimulated HeLa cell lysate, in 40 µL of 1X MLB (Catalog 20-168) containing, 1 &micor;g/mL leupeptin, 1µg/mL aprotinin, 1 µg/mL pepstatin, 1mM PMSF.

Preparation Note

Stable for 6 months at -80C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap. Use immediately in assay following thawing as Ras GTP form can rapidly hydrolyze to the inactive Ras GDP form.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS07
• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• signalword: Danger
• hcodes: H319,EUH430
• pcodes: P201 - P202 - P273 - P391 - P405 - P501
• Hazard Classifications: ED ENV 1 - Eye Irrit. 2