1.10549

Listeria Enrichment Broth

GranuCult^® plus, according to FDA IDF-FIL, granular, for Listeria spp., pack of 5 kg

• Synonym(s): Listeria Enrichment broth, LEB
• NACRES: NA.74
• UNSPSC Code: 41106213
• Manufacturer/tradename: GranuCult^® plus
• Ph: 7.1-7.5 (30 °C, 36.1 g/L in H₂O, after autoclaving)
• Form: granular
• Suitability: Listeria spp.
• Application(s): food and beverages; microbiology

Properties

• reg. compliance: according to FDA IDF-FIL
• Quality Segment: 200
• sterility: non-sterile
• form: granular
• packaging: pack of 5 kg
• manufacturer/tradename: GranuCult^® plus
• technique(s): microbiological culture: suitable
• pH: 7.1-7.5 (30 °C, 36.1 g/L in H₂O, after autoclaving)
• solubility: 36.1 g/L
• application(s): food and beverages; microbiology
• storage temp.: 2-30°C
• suitability: Listeria spp.

Description

General description

The Granucult^® Listeria Enrichment Broth formulation is a modified Tryptic Soy (CASO) Broth with an additional 6 g/liter of yeast extract. The inhibition of undesired accompanying flora is achieved by the addition of acriflavin HCl, cycloheximide, and nalidixic acid. In contrary to Listeria Enrichment Broth (Base) (1.11951), this broth already contains antibiotic substances.

Application

Granucult^® Listeria Enrichment Broth is intended for the selective enrichment of Listeria.

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 7.1 - 7.5
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): 10 - 100
Inoculum on reference medium (Listeria monocytogenes ATCC 19114 (WDCM 00110)): 10 - 100
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): 10 - 100
Inoculum on reference medium (Listeria innocua ATCC 33090 (WDCM 00017)): 10 - 100
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≥ 1E+04 CFU/ml
Colony count (Listeria monocytogenes ATCC 19114 (WDCM 00110)): ≥ 1E+04 CFU/ml
Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≥ 1E+04 CFU/ml
Colony count (Listeria innocua ATCC 33090 (WDCM 00017)): ≥ 1E+04 CFU/ml
Colony count (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≤ 1000 CFU/ml
Incubation: 24 hrs.; 30 °C; aerobically.

Other Notes

Replaces: 1.10549.5007

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3