1.08380

2,3,5-Triphenyltetrazolium chloride

Color indicator. Supplement in culture media for the microbiological analysis of food and water samples

• Synonym(s): 2,3,5-Triphenyltetrazolium chloride, TTC, TT, TPTZ, TTZ, Tetrazolium Red
• Empirical Formula (Hill Notation): C₁₉H₁₅ClN₄
• CAS Number: 298-96-4
• Molecular Weight: 334.80
• NACRES: NA.74
• UNSPSC Code: 41106212
• Beilstein/REAXYS Number: 3923356
• MDL number: MFCD00011963

Properties

• Quality Segment: 200
• assay: ≥98% (calculated on dried substance, perchloric acid titration)
• form: solid
• pH: 3.7 (20 °C, 10 g/L in H₂O)
• mp: 243 °C (decomposition)
• solubility: 150 g/L
• bulk density: 230 kg/m³
• application(s): microbiology
• storage temp.: 2-30°C
• SMILES string: [Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4
• InChI: 1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1
• InChI key: PKDBCJSWQUOKDO-UHFFFAOYSA-M

Description

General description

2,3,5-triphenyltetrazolium chloride (TTC) is a dye largely used for enumeration of microbial colonies in solid culture media, being a key component of the dry rehydratable film system used for microbiological analysis of food. This dye is colorless in the oxidized form and red when reduced by microorganisms, due to formation of formazan. Live microorganisms reduce TTC by enzymatic action, forming red color formazan that is kept inside granules in the cells.

Application

2,3,5-triphenyltetrazolium chloride (TTC) is an indicator recommended for the microbiological analysis of food.

Analysis Note

Assay (perchloric acid titration, calculated on dried substance): ≥ 98 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ_max. (water): 245 - 248 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0,01 g/l; water; calculated on dried substance): ≥ 770
TLC-Test: passes test
Loss on drying (70 °C; ≤ 1 hPa): ≤ 0.5 %
Suitability for testing the germinability of seeds: passes test
Suitability for microbiology: passes test

Safety Information

• pictograms: GHS02,GHS07
• signalword: Danger
• hcodes: H228 - H315 - H319
• pcodes: P210 - P240 - P241 - P264 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Sol. 1 - Skin Irrit. 2
• Storage Class: 4.1B - Flammable solid hazardous materials
• wgk: WGK 3