1.01656

Benzonase^® purity grade II (>90%) for biotechnology

• Synonym(s): Endonuclease from Serratia marcescens
• CAS Number: 9025-65-4
• UNSPSC Code: 12352202
• MDL number: MFCD00131010
• EC Number: 3.1.30.2 (BRENDA, IUBMB)

Properties

• biological source: Serratia marcescens
• Quality Level: 100
• recombinant: expressed in E. coli
• sterility: non-sterile
• assay: ≥90% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: ≥250 units, pH 8.0, 37 °C
• concentration: ≥250 units/μL
• impurities: ≤10 CFU in 100 000 U Colony count (Yeasts and moulds), ≤10 CFU in 100 000 U Colony count (aerobic bacteria), not detectable Proteases
• color: clear, colorless
• pH: 8 (25 °C in H₂O)
• application(s): pharma/biopharma processes
• foreign activity: foreign activity ≥1.0 x 10^6 units (calc. on protein)
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Our comprehensive portfolio of downstream process chemicals not only provides biopharmaceutical manufacturers with high-quality raw materials for production of classical and novel therapies, but also helps them get to market faster and simplify regulatory challenges. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

We offer a range of high quality critical raw materials plus the documentation and expertise to help our customers meet stringent regulatory requirements. Our SAFC^® portfolio of high-quality products withstands strict quality control procedures.

Application

Benzonase^® purity grade II (>90%) for biotechnology is the technical grade in our Benzonase^® product portfolio. All Benzonase^® endonuclease products share the same amino acid sequence (proven by LC-MS/MS mass spectrometry), robustness and activity. The technical grade can be used to optimize the production / testing process before using one of our GMP grade during verification and manufacturing process.

Used for the removal of nucleic acid from protein samples.

Biochem/physiol Actions

Digests native or heat-denatured DNA and RNA.

Packaging

500,000 Units5,000,000 Units

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1