1.00191

Bismuth Sulfite (BS) Agar

acc. to Wilson and Blair, NutriSelect^® Prime, powder, pack of 500 g, suitable for microbiology

• Synonym(s): BSA, WILSON BLAIR Agar, Wismuth sulfite agar
• NACRES: NA.74
• UNSPSC Code: 41106213
• Manufacturer/tradename: NutriSelect^® Prime
• Agency: ISO 6579-1, USP 31
• Ph: 7.7±0.2 (25 °C)
• Form: powder
• Suitability: selective and differential for enterobacteriaceae: Salmonella spp.
• Application(s): food and beverages; pharmaceutical, microbiology

Properties

• Quality Segment: 200
• agency: ISO 6579-1, USP 31
• description: FDA-BAM
• form: powder
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Prime
• technique(s): microbiological culture: suitable
• pH: 7.7±0.2 (25 °C)
• application(s): food and beverages; pharmaceutical, microbiology
• storage temp.: 2-8°C
• suitability: selective and differential for enterobacteriaceae: Salmonella spp.

Description

General description

NutriSelect^® Bismuth Sulfite (BS) Agar introduced by Wilson-Blair (1927) is specifically designed for the isolation and differentiation of Salmonella spp from food, animal feed and other materials precisely when the detection of Salmonella enterica subspecies enterica serovars Typhi and Paratyphi is of concern.
Colonies of H₂S positive Salmonella exhibit blackening due to the formation of iron sulfide. The media selectivity is enhanced by the presence of brilliant green and bismuth sulfite which inhibit the growth of gram-positive bacteria and coliforms in the sample.
Peptone, meat extract and glucose allow good growth. Disodium phosphate acts as a buffer to maintain pH and agar is the solidifying agent.

Application

NutriSelect^® Bismuth Sulfite (BS) Agar is used to isolate and differentiate Salmonella spp. from food animal feed and other materials. The medium is used to selectively grow the species when S. typhi and S. paratyphi are expected.

Analysis Note

Salmonella colonies display blackening after 18 hrs of incubation.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 11 - Combustible Solids