09-893

Anti-BAF (BANF1) Antibody

serum, from rabbit

• Synonym(s): 53 kDa BRG1-associated factor A, Actin-related protein Baf53a, BAF complex 53 kDa subunit, BRG1-associated factor, INO80 complex subunit K, actin-like 6A, actin-related protein 4, hArpN beta
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 1

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat, mouse, human
• technique(s): western blot: suitable
• NCBI accession no.: NP_003851
• UniProt accession no.: O96019
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... BANF1(8815)

Description

General description

Barrier-to-auto integration factor (BAF or BANF1) plays fundamental roles in nuclear assembly, chromatin organization, gene expression and development. BAF may potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. BAF contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. BAF is exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. BAF is found in both the nucleus and cytoplasm and is significantly enriched at the nuclear inner membrane, diffused throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase.

~10 kDa

Immunogen

KLH-conjugated linear peptide corresponding to human BAF (BANF1).

Application

Use Anti-BAF (BANF1) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect BAF (BANF1) also known as 53 kDa BRG1-associated factor A.

Western Blot Analysis: A 1:1,000 dilution from a previous lot detected BAF in C2C12, Hek293, HeLa, HepG2, Huvec, Jurkat, L6, NIH/3T3, PC12, PC3, and RAW264.7 cell lysates.

Biochem/physiol Actions

This antibody recognizes BAF (BANF1).

Analysis Note

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected BAF in 10 µg of HeLa cell lysate.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1