07-442

Anti-trimethyl-Histone H3 (Lys9) Antibody

Upstate^®, from rabbit

• Synonym(s): H3K9me3, Histone H3 (tri methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human, mouse, rat, chicken
• Application: ChIP, DB, ICC, WB, inhibition assay, multiplexing
• Citations: 601

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse, rat, chicken
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable (ChIP-seq), dot blot: suitable, immunocytochemistry: suitable, inhibition assay: suitable (peptide), multiplexing: suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_003493
• UniProt accession no.: Q16695
• shipped in: dry ice
• target post-translational modification: trimethylation (Lys9)
• Gene Information: human ... H3C1(8350) ; mouse ... H3C1(360198) ; rat ... H3C1(679994)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

17 kDa

Immunogen

Epitope: Lys9

KLH-conjugated, 2X-branched synthetic peptide containing the sequence …AR[me3K]ST… in which me3K corresponds to trimethyl-lysine at residue 9 of human histone H3.

Application

Anti-trimethyl-Histone H3 (Lys9), Cat. No. 07-442 is a rabbit polyclonal antibody that detects Histone H3 (Met3-Lys9) and is tested for use in Western Blotting, Dot Blot, Peptide Inhibition Assay, and Immunocytochemistry.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Tested Applications

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Histone H3 (Met3-Lys9) in Mouse Embryo Fibroblasts (MEF).

Peptide Inhibition Assay: Target band detection in acid extract of HeLa cells was prevented by pre-blocking of a representative lot with the immunogen Histone H3 (Met3-Lys9) peptide, but not the corresponding unmodified Histone H3 peptide.

Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected trimethyl-Histone H3 (Lys9) peptide, but did not detect di-methyl Histone H3 (Lys9) peptide, mono-methyl Histone H3 (Lys9) peptide, nor unmodified Histone H3 (1-19) peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Expected to cross-react with chicken, mouse and rat.

Trimethyl-histone H3 (Lys9). Broad species cross-reactivity expected.

Physical form

Format: Purified

Protein A chromatography

Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.

Preparation Note

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Acid-extracted proteins from HeLa cells.

Evaluated by Western Blotting in HeLa cell acid extract.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Histone H3-Lys9me3 in HeLa cell acid extract, but did not detect unmodified recombinant Histone H3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids