07-396

Anti-C-RAF antibody

Upstate, rabbit polyclonal

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: human
• Application: IP, WB
• Citations: 19

Properties

• Product Name: Anti-Raf-1 Antibody, Upstate^®, from rabbit
• biological source: rabbit
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_002880
• UniProt accession no.: P04049
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RAF1(5894)

Description

General description

70kDa

Immunogen

peptide corresponding to amino acids 637-648 of human Raf-1.

Application

Anti-Raf-1 Antibody detects level of Raf-1 & has been published & validated for use in IP & WB.

Research Category
Signaling

Research Sub Category
MAP Kinases

Biochem/physiol Actions

Raf-1

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%

Format: Purified

Protein A purified

Preparation Note

2 years at -20degC

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot in RIPA lysates from 3T3/A31 cell lysates

Other Notes

Replaces: 04-412

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2