07-215

Anti-dimethyl-Histone H3 (Arg26) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): H3R26me2, Histone H3 (di methyl R26), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: vertebrates, Tetrahymena sp., Xenopus, human
• Application: WB, multiplexing
• Citations: 11

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: vertebrates, Tetrahymena sp., Xenopus, human
• technique(s): multiplexing: suitable, western blot: suitable
• NCBI accession no.: NP_003484.1
• UniProt accession no.: Q16695
• shipped in: wet ice
• target post-translational modification: dimethylation (Arg26)
• Gene Information: human ... H3C1(8350)

Description

General description

17kDa

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen

Epitope: Dimethylated Histone H3 (Arg26)

KLH-conjugated synthetic peptide (ATKAA(di-Me)RKSAPA-C) corresponding to amino acids 21-31 of Human Histone H3.

Application

Research Sub Category
Histones

Anti-dimethyl-Histone H3 (Arg26) Antibody is a rabbit polyclonal antibody for detection of dimethyl-Histone H3 (Arg26) also known as H3R26me2, Histone H3 (di methyl R26) & has been validated in WB, Mplex.

Research Category
Epigenetics & Nuclear Function

SNAP I.D. (Western Blotting Technique):
A previous lot of this antibody worked successfully using the SNAP I.D. western blotting device and technique.

Biochem/physiol Actions

Recognizes CARM1 in vitro methylated recombinant Histone H3. The immunizing sequence is conserved from Tetrahymena through human therefore a broad cross reactivity is expected.

Recognizes dimethylated Histone H3 at and around arginine 26, MW ~17 kDa. Possible slight reactivity with monomethyl histone H3 (Arg26).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl, with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
CARM1 in vitro methylated recombinant Histone H3.

Evaluated by western blot on untreated and CARM1 in vitro methylated recombinant Histone H3.

Western Blot Analysis: 1:8000 dilution of this antibody detected dimethyl-Histone H3 (Arg26) in untreated and CARM1 in vitro methylated recombinant Histone H3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1