07-1735

Anti-HuR Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): ELAV-like 1, Hu antigen R, Hu-antigen R, embryonic lethal, abnormal vision, drosophila, homolog-like 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IF, WB
• Citations: 5

Properties

• Quality Segment: 100
• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, rat, mouse
• technique(s): immunofluorescence: suitable, western blot: suitable
• NCBI accession no.: NP_001410.2
• UniProt accession no.: Q15717
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ELAVL1(1994) ; mouse ... Elavl1(15568) ; rat ... Elavl1(363854)

Description

General description

36 kDa

HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. Some of its targets include cFos, IL-3, and BRCA1 mRNAs. HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types. It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK.

Immunogen

Epitope: N-terminus

Synthetic peptide corresponding to the N-terminus of human HuR.

Application

Immunofluorescent ICC Analysis:

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

RNA Binding Protein (RBP)

Use Anti-HuR Antibody (Rabbit Polyclonal Antibody) validated in WB, IF to detect HuR also known as ELAV-like 1, Hu antigen R.

Biochem/physiol Actions

Detects HuR

Other species have not been tested.

Physical form

Affinity purified polyclonal precipitated in ammonium sulfate.

Rabbit polyclonal precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Analysis Note

Control
Jurkat cell lysate

Western Blot Analysis:
The antibody detected HuR at 1:500-1:1,000 dilutions in Jurkat cell lysate resolved via SDS-PAGE and transferred to PVDF.

Other Notes

Replaces: AB5831

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable