07-1341

Anti-PHLPP1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): PH domain and leucine rich repeat protein phosphatase, PH domain leucine-rich repeat protein phosphatase, Pleckstrin homology domain-containing family E protein 1, SCN circadian oscillatory protein, Suprachiasmatic nucleus circadian oscillatory protein
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: conjugate
• Clone: polyclonal
• Application: ICC, WB
• Citations: 19

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: conjugate
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• purified by: affinity chromatography
• species reactivity: rat, mouse, human
• technique(s): immunocytochemistry: suitable, western blot: suitable
• immunogen sequence: KLH-conjugated linear peptide corresponding to the C-terminus of PHLPP1.
• NCBI accession no.: NP_919431
• UniProt accession no.: O60346
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... PHLPP1(23239)

Description

General description

134 kDa Observed at 110 kDa

PHLPP1 is the protein phosphatase responsible for dephosphorylation of the Akt (Ser473) site. Akt (Ser473) is the TORC2 (mTOR) phosphorylation activation site.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of PHLPP1.

Application

Anti-PHLPP1 Antibody detects level of PHLPP1 & has been published & validated for use in WB & IC.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Biochem/physiol Actions

This antibody recognizes the C-terminal region of PHLPP1.

Physical form

Affinity purified

Purified rabbit serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.

Preparation Note

Maintain refrigerated at 2-8°C for 1 year from date of receipt.

Analysis Note

Control
Human brain tissue lysate

Evaluated by Western Blot in Human brain tissue lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected PHLPP1 in Human brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable