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Anti-Pin1 Antibody

Name: Anti-Pin1 Antibody
Brand: MM
Price: 11000 CZK
Availability: InStock

Upstate^®, from rabbit

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Pack Size	SKU	Availability	Price
100 μg		Available to ship TODAYfromAreál Kühne+Nagel spol. s r.o.	CZK 11,000.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IP, WB

Citations:

CZK 11,000.00

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Properties

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, rat, mouse

manufacturer/tradename

Upstate^®

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG

NCBI accession no.

NM_006221

UniProt accession no.

Q13526

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... UBL5(59286)

Description

General description

18kDa

Pin1 is a peptidyl-prolyl isomerase (PPI) that targets phosphorylated Ser or Thr residues followed by a Pro (S/T-P). Isomerization of phosphorylated Ser or Thr residues may alter protein confirmation and, subsequently, modify activity. Pin1 is overexpressed in many human breast cancers, and has been shown to modify numerous proteins including p53,
NF-κB, c-Jun, cyclin D1, and β-catenin.

Immunogen

Full-length His tagged human Pin-1

Application

Anti-Pin1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Pin1 & has been validated in IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

Pin1

Physical form

Format: Purified

Presentation: 100 µg of protein A purified rabbit IgG in 100 µL of 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% Sodium Azide and 30% glycerol. Liquid at -20°C.

Protein A chromatography

Preparation Note

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Routinely evaluated by immunoblot on RIPA lysates from mouse 3T3/A31 cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	MABC1564	HPA023139	MABC1190
Sigma-Aldrich 07-091 Anti-Pin1 Antibody	Sigma-Aldrich MABC1564 Anti-Pin1 Antibody, clone 5B4.1	Sigma-Aldrich HPA023139 Anti-PINX1 antibody produced in rabbit	Sigma-Aldrich MABC1190 Anti-Bin1 Antibody, clone 2F11
species reactivity human, rat, mouse	species reactivity human, rat	species reactivity human	species reactivity mouse, rat, chicken, human
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form purified immunoglobulin
clone polyclonal	clone 5B4.1, monoclonal	clone polyclonal	clone 2F11, monoclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source mouse	biological source rabbit	biological source mouse
technique(s) immunoprecipitation (IP): suitable, western blot: suitable	technique(s) immunohistochemistry: suitable (paraffin), western blot: suitable	technique(s) immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:50-1:200	technique(s) immunohistochemistry: suitable (paraffin), western blot: suitable

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Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Anti-Diabetic Atherosclerosis by Inhibiting High Glucose-Induced Vascular Smooth Muscle Cell Proliferation via Pin1/BRD4 Pathway.

Yuansheng Wu et al.

Oxidative medicine and cellular longevity, 2020, 4196482-4196482 (2020-08-11)

Diabetic Apoe-/- mice induced by streptozotocin were treated with vehicle, the Pin1 inhibitor juglone, or the BRD4 inhibitor JQ1 for 3 weeks. VSMCs were pretreated with juglone, JQ1, or vehicle for 45 min, and then exposed to high glucose for 48 h.

Depletion of mitochondrial methionine adenosyltransferase α1 triggers mitochondrial dysfunction in alcohol-associated liver disease.

Lucía Barbier-Torres et al.

Nature communications, 13(1), 557-557 (2022-01-30)

MATα1 catalyzes the synthesis of S-adenosylmethionine, the principal biological methyl donor. Lower MATα1 activity and mitochondrial dysfunction occur in alcohol-associated liver disease. Besides cytosol and nucleus, MATα1 also targets the mitochondria of hepatocytes to regulate their function. Here, we show

A human peptidyl-prolyl isomerase essential for regulation of mitosis.

K P Lu et al.

Nature, 380(6574), 544-547 (1996-04-11)

The NIMA kinase is essential for progression through mitosis in Aspergillus nidulans, and there is evidence for a similar pathway in other eukaryotic cells. Here we describe the human protein Pin1, a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA.