06-599

Anti-acetyl-Histone H3 Antibody

from rabbit

• Synonym(s): H3 histone family, member T, histone 3, H3, histone cluster 3, H3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: mouse, rat, human
• Application: ChIP, ICC, WB
• Citations: 2391

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, rat, human
• technique(s): ChIP: suitable (ChIP-seq), immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_003484.1
• UniProt accession no.: Q16695
• shipped in: wet ice
• target post-translational modification: acetylation (not specified)
• Gene Information: human ... H3C1(8350) ; mouse ... H3C1(360198) ; rat ... H3C1(679994)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.

~ 17 kDa observed

Immunogen

Epitope: N-terminus.

Linear peptide corresponding to human Histone H3 acetylated at the N-terminus.

Application

Detect acetyl-Histone H3 with Anti-acetyl-Histone H3 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB, ICC, ChIP, ChIP-seq.

Immunocytochemistry Analysis: 1:500 dilution of a representative lot of this antibody detected acetylated Histone H3 in NIH/3T3 and A431 cells.

Chromatin Immunoprecipitation: This antibody has been shown to work for chIP using HeLa extracted chromatin and 5 mg of this antibody.

ChIP-Seq: This antibody has been shown to work for ChIP-Seq in Kanai, A., et. al. (2011). DNA Research 18:379-392.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Biochem/physiol Actions

Broad species cross-reactivity expected based on sequence similarity.

Recognizes Histone H3 acetylated at the N-terminus.

Physical form

Format: Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa acid extract, sodium butyrate treated HeLa cells, and recombinant Histone H3

Evaluated by Western Blot in HeLa acid extract, sodium butyrate treated HeLa cells, and recombinant Histone H3.

Western Blot Analysis: 0.05 µg/mL of this antibody detected Histone H3 on 10 µg of HeLa acid extract, sodium butyrate treated HeLa cells, and recombinant Histone H3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids