06-284

Anti-Nitrotyrosine Antibody

Upstate^®, from rabbit

• Synonym(s): Anti-Nitrotyrosine, Detection Antibody, Nitrotyrosine Detection Antibody
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• Application: ICC, IHC, IP, WB
• Citations: 378

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity (predicted by homology): all
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... NOS1(4842)

Description

General description

Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others.

Predicted MW for nitrated-BSA: 85 kDa

Immunogen

Nitrated KLH

Application

• Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected in fixed and permeabilized A-431 cells treated with 5mM peroxynitrite for 15 minutes.
• Immunohistochemistry Applications: A representative lot detected Nitrotyrosine in human colon cancer, human Alzheimer′s brain and human diabetic kidney tissue sections.
• Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Research Sub Category
Neurotransmitters & Receptors

Neurodegenerative Diseases

This rabbit polyclonal Anti-Nitrotyrosine, Cat. No. 06-284 is tested for use in Western Blotting, Immunohistochemistry and Immunocytochemistry, for the detection of Nitrotyrosine.

Biochem/physiol Actions

Recognizes proteins which are nitrated.

Physical form

Format: Purified

ImmunoAffinity Purified

Purified Rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, 0.05% sodium azide, and 10 mg/mL BSA

Preparation Note

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

routinely evaluated by immunoblot on nitrotyrosine immunoblotting controls (Catalog #12-354)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable