05-858

Anti-Histone H4 Antibody, pan, clone 62-141-13, rabbit monoclonal

clone 62-141-13, Upstate^®, from rabbit

• Synonym(s): H4, Histone H4, H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 62-141-13, monoclonal
• Application: ChIP, WB, multiplexing
• Citations: 154

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 62-141-13, monoclonal
• species reactivity: chicken, Saccharomyces cerevisiae, vertebrates, yeast, human
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable (ChIP-seq), multiplexing: suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_003529
• UniProt accession no.: P62805
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: Saccharomyces cerevisiae ... Hhf1(852294) ; human ... H4C1(8359)

Description

General description

10 kDa

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

This antibody recognizes histone H4 independant of any post-translational modifications.

Immunogen

Epitope: a.a. 25-28

KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28.

Application

Anti-Histone H4 Antibody, pan, clone 62-141-13 is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex, ChIP-seq.

Epitope Mapping (using Luminex^®):
1:250-1:4,000 serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the amino-terminus of Histone H4 conjugated to Luminex microspheres. The antibody recognized both modified and unmodified peptides (See Figure B Page 2).

Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Biochem/physiol Actions

Broad species cross-reactivity expected based on sequence homology.

Recognizes Histone H4, Mr 10 kDa.

Physical form

Format: Purified

Protein A purified

Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated).

Routinely evaluated by western blot.

Western Blot Analysis:
A 1:30,000-1:100,000 dilution of this lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Luminex is a registered trademark of Luminex Corp

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable