05-677

Anti-phospho-Src (Tyr416) Antibody, clone 9A6

clone 9A6, Upstate^®, from mouse

• Synonym(s): proto-oncogene tyrosine-protein kinase SRC, protooncogene SRC, Rous sarcoma, tyrosine kinase pp60c-src, tyrosine-protein kinase SRC-1, v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog, v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homo
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 9A6, monoclonal
• Species reactivity: mouse, human
• Application: IP, WB
• Citations: 38

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9A6, monoclonal
• species reactivity: mouse, human
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG3
• NCBI accession no.: NM_005417
• UniProt accession no.: P12931
• shipped in: wet ice
• target post-translational modification: phosphorylation (pTyr416)
• Gene Information: human ... SRC(6714)

Description

General description

60 kDa

Specific src isoform is unknown

Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.

Immunogen

KLH-conjugated, synthetic peptide containing phosphotyrosine in the sequence context corresponding to tyrosine 416 of avian Src. Clone 9A6.

Application

Detect phospho-Src (Tyr416) using this Anti-phospho-Src (Tyr416) Antibody, clone 9A6 validated for use in WB & IP.

Immunoprecipitation/Immunoblot Analysis: 4 μg/mL of a previous lot detected phospho-Src immunoprecipitated from 3T3 cell lysates with 10 μg of anti-Src (Catalog # 05-184), but not after phosphatase treatment with YOP.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Biochem/physiol Actions

Recognizes phospho-Src, Mr 60kDa. The antibody does not crossreact with the nonphosphorylated form of Src or with unrelated phosphorylation sites. Predicted to recognize all Src family members phosphorylated at the tyrosine corresponding to Tyr416 of avian Src.

Physical form

Format: Purified

Purified mouse monoclonal IgG3 lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 mL H2O (15 min, RT). Lyophilized. Store at -20ºC.

Thiophilic adsoption and size exclusion chromatography

Preparation Note

Stable for 1 year at from date of receipt.

Analysis Note

Control
Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein

Evaluated by immunoprecipitating Src from 3T3 cell lysates with anti-Src (Catalog #05-184), but not after phosphatase treatment with YOP.

Src Autophosphorylation/Western Blotting Analysis: 0.5-2 μg/mL of this antibody strongly detected recombinant Src (Catalog # 14-326) after incubation with Mg/Mn/ATP. Phospho-specificity was confirmed by dephosphorylation of the Src protein with λ-phosphatase.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3