04-772

Anti-Src Antibody, CT, clone NL19 | 04-772

clone NL19, Upstate^®, from rabbit

• Synonym(s): Proto-oncogene tyrosine-protein kinase SRC, Protooncogene SRC, Rous sarcoma tyrosine kinase pp60c-src, Tyrosine-protein kinase SRC-1, v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: NL19, monoclonal
• Application: IP, WB
• Citations: 5

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: NL19, monoclonal
• species reactivity: mouse, human, rat
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_005417
• UniProt accession no.: P12931
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... SRC(6714)

Description

General description

SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.

~53-62kDa

Immunogen

KLH conjugated synthetic peptide (CKVADFGLARLIEDNEYTARQGA) of Src (avian sarcoma virus), residues 400-422. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck.

Application

Western Blot Analysis: A 1:2000 to 1:8000 dilution of this lot detected Src in RIPA whole cell lysates from human A431 cells. Immunoprecipitation: 5-20 μL of a previous lot immunoprecipitated Src from 500 μg of A431 RIPA lysate.

Biochem/physiol Actions

Predicted to cross react with frog and chicken. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck.

Src-tyrosine kinase family

Physical form

Format: Purified

Analysis Note

Control
RIPA whole cell lysates from human A431, Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells Included Positive Antigen Control: Catalog # 12-301, Non-Stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot on RIPA whole cell lysates from human A431 and Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells

Other Notes

Replaces: 05-772

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable