05-666

Anti-BRCA2 Antibody, clone 5.23

clone 5.23, Upstate^®, from mouse

• Synonym(s): Anti BRCC2, Anti-BROVCA2, Anti-FACD, Anti-FAD, Anti-FAD1, Anti-FANCD, Anti-FANCD1, Anti-GLM3, Anti-PNCA2, Anti-XRCC11
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 5.23, monoclonal
• Application: ICC, WB
• Citations: 15

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5.23, monoclonal
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_000059
• UniProt accession no.: P51587
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... BRCA2(675)

Description

General description

390kDa

Immunogen

GST fusion protein to residues 2959-3418 of human BRCA2 protein

Application

Detect BRCA2 with Anti-BRCA2 Antibody, clone 5.23 (Mouse Monoclonal Antibody), that has been shown to work in WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

BRCA2

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 0.05% sodium azide.

Format: Purified

Protein G Chromatography

Preparation Note

Store at 2-8°C. Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C.

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

routinely evaluated by immunoblot on in HeLa nuclear extract

Other Notes

Due to license agreement restrictions, this product cannot be purchased for resale.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1