05-531

Anti-His Tag Antibody

clone 4D11, mouse monoclonal, Upstate

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43
• Clone: 4D11, monoclonal
• Technique(s): immunocytochemistry: suitable, western blot: suitable
• Application: ICC, WB
• Citations: 13

Properties

• Product Name: Anti-Histidine Tagged Antibody, clone 4D11, clone 4D11, Upstate^®, from mouse
• biological source: mouse
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: 4D11, monoclonal
• species reactivity (predicted by homology): all
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG2aκ
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... HRG(3273)

Description

General description

The apparent molecular weight is related to the tagged protein.

Immunogen

N-terminal His-tagged and truncated TRAP protein corresponding to amino acids 26-248 from P. falciparum expressed in E coli.

Application

This Anti-Histidine Tagged Antibody (anti His antibody) clone 4D11 is validated for use in IC, WB for the detection of Histidine Tagged.

Biochem/physiol Actions

Recognizes tag sequence HHHHHHGS on either the N- or C-terminus of recombinant proteins; GS amino acids are required for recognition of 6His tag

Physical form

Format: Purified

Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, and 0.07% sodium azide.

Analysis Note

Control
Cells expressing the His-tag

routinely evaluated by immunoblot on recombinant N-terminal and C-terminal His-tagged proteins

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable